84-2655-73　［取扱停止］VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF) (MaxLight 405) 100ul　V2110-09A-ML405

特徴

• MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.
• Applications:Suitable for use in Neutralization, FLISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Formalin-fixed paraffin embedded): 5-15ug/ml with a 30 minute incubation at RT or an overnight incubation at 4ºC
• Neutralization: 0.05-0.1ug/ml yields one-half maximal inhibition [ND50] of the biological activity (10ng/ml)
• FLISA (Indirect amd Sandwich): 0.5ug/ml (100ul/well).
• Used in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well of recombinant hVEGF
• Western Blot: 0.1-0.2ug/ml.
• Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™405 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Country of Origin:USA
• Note: Applications are based on unconjugated antibody.