Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine.
Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines.
TH has a large molecular diversity, resulting from differential splicing of its mRNA, which is tissue specific and might result in long term changes in activity of the enzyme and the availability of neurotransmitter at various synapses.
The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia.
Parkinson's disease is also considered a TH deficiency, as low dopamine levels are a consistent neurochemical abnormality.
This antibody is an excellent marker for dopaminergic and noradrenergic neurons.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes rat tyrosine hydroxylase. Species Crossreactivity: mouse, human, monkey. Detects all mammalian forms of TH tested, some non-mammalian, and some invertebrate forms of TH.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Ma Mk Mo Rt
Immunogen：Native rat tyrosine hydroxylase purified from pheochromocytoma.
