One family of inhibitory axon guidance molecules is the semaphorins.
The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets.
SEMA 3B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:40Western Blot: 1:5000Immunohistochemistry(Paraffin): 1:100Immunocytochemistry: 1:40Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity:Recognizes human SEMA3B. Species Crossreactivity: rat, mouse, primate, bovine, canine
Isotype:IgG
Calc Applications Abbrev:IC IF IHC WB
Calc Crossreactivity:Bo Ca Mk Mo Rt
Immunogen:Synthetic peptide corresponding to aa100-200 at the internal portion of human SEMA3B.