Pin-1 is a the peptidylprolyl cis/trans isomerase enzyme which is responsible, as its name suggests, for flipping the proline ring from the cis to the trans conformation.
Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease.
Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau.
Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation.
Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain.
Pin1 could be pivotal for maintainance of normal neuronal function and preventing age-dependent neurodegeneration. It is also heavily upregulated in tumor cells, so that antibodies to this protein can be used as tumor markers.
Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:1000 Western Blot: 1:10,000 Immunocytochemistry(ABC): 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：chicken
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 10mM sodium azide.
Specificity：Recognizes Pin-1. Species Crossreactivity: human
Isotype：IgY
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length Pin-1, expressed in E. coli.
