Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells.
In the electron microscope neurofilaments appears as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides.
They are found particularly abundantly in axons of large projection neurons.
Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:20,000 Immunohistochemistry: 1:500-1:1000 Immunocytochemistry: 1:5000 (ABC or other enzyme linked procedure) Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid.
Specificity：Recognizes rat NF-M. Species Crossreactivity: human, mouse, feline, chicken
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Ch Fe Hu Mo Rt
Immunogen：Recombinant fusion protein corresponding to the extreme C-terminal segment of rat NF-M.
