mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments.
The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kD protein isolated originally from the jellyfish Aequoria victoria.
The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
DsRed is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome.
Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which has an excitation maximum at 587nm and and emission maximum at 610nm.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 10mM sodium azide.
Specificity：Recognizes mCherry. Species Crossreactivity: mammalian
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Ma
Immunogen：Full length recombinant protein corresponding to mCherry fused to His-tag, expressed in E. coli.
