HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis.
This occurs as a posttranslational modification by prolyl hydroxylation. It is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits.
Both subunits are constantly translated.
However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes.
Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity.
Therefore, the concentration of HIF-1 alpha increases in the cell.
In contrast, HIF-1 beta remains stable under either condition.
HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500 Immunohistochemistry (Paraffin): 1:150-1:300Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human HIF-1 alpha. Species Crossreactivity: primate
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Genomic sequence made to an internal portion of the human protein (aa400-550). Species sequence homology: canine and bovine (95%), porcine (93%), mouse and rat (88%)
