Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system.
Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation.
In the central nervous system, Histamine is putative neurotransmitter.
In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.
The Histamine antiserum has a sensitivity level capable of detecting the low level Histamine contents of the brain.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:500-1:1000 (in PBS/0.3% Triton X-100 using Cy3 technique)Immunohistochemistry: 1:4000-1:6000 (in PBS/0.3% Triton X-100 using Bn/Av-HRP technique)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in 0.09% sodium azide.
Specificity：Recognizes Histamine. No cross reactivity with L-Histidine or L-Histidine-containing peptides usch as LH-RH.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Rt
Immunogen：Synthetic histamine corresponding to Histamine, coupled to KLH with carbodiimide (CDI) linker.
