GAP-43 (growth associated protein 43kD; also known as neuromodulin) is a 24kD, neuron growth cone-associated member of the neuromodulin family.
Apparently due to its tertiary structure, it runs anomalously at 43kD on SDS-polyacrylamide gel.
Rat GAP-43 is a 226aa protein that contains an N-terminal lipid binding motif, an 11aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa38-48), and a C-terminus with four potential serine phosphorylation sites.
The molecule may utilize the IQ domain to bind calmodulin.
Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction.
Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.
GAP43 is a proven marker for developing and regenerating neurons, and to a lesser extent, reactive glial cells.
It is used widely to specifically label injured neurons and to score neuronal regeneration.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:1000Western Blot: 1:5000-1:10,000, recognizes a band at ~43kDOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid.
Specificity：Recognizes GAP43. Species Crossreactivity: human, rat, mouse, bovine
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Peptide corresponding to the C-terminal of rat and mouse GAP43, KEDPEADQEHA, N-terminal Cysteine residue, coupled to KLH.
