Fox3 is one of a family of 3 mammalian Fox homologues.
Fox was discovered in C.
elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X".
The Fox protein and its 3 mammalian homologues are all about 46kD proteins each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70aa structure consisting of 4 beta strands and two alpha-helices.
An alternate name for Fox 3 is hexaribonucleotide binding protein 3, and these proteins are believed to have a role in the regulation of mRNA splicing.
Much interest has focused on Fox3 as this protein corresponds to NeuN, a neuronal nuclear antigen.
NeuN antibody has become very widely used as a robust marker of neurons and neuronal stem cells.
Fox3 is therefore a protein which has a funciton in RNA splicing and is expressed heavily and specifically in neuronal nuclei.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:1000Western Blot: 1:10,000Immunohistochemistry (frozen and fixed tissue): 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes human Fox3. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to aa1-100 of human FOX3 at N-terminal, expressed in E. coli.
