Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis.
During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor b (TGFb) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment.
In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation.
Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.
Applications:Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:20-1:100Flow Cytometry: 1:50Western Blot: 1:1000-1:2000Immunocytochemistry: 1:20-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Cripto1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：FC IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa100-150 at the internal portion of human Cripto1.
