Bmi1 induces telomerase activity and immortalizes human mammary epithelial cells.
Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.
It has also been termed an oncogene with aberrant expression in various carcinomas.
It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors.
Ablation of Bmi1 is required for short term survival of cancer cells
In addition, Bmi1 expression promotes HSC self-renewal.
These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:50-1:200, a band is seen ~37kDImmunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Bmi1. Species Crossreactivity: rat, mouse, rabbit, chicken, primate, feline, zebrafish
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Ch Fe Hu Mk Mo Rb Rt Ze
Immunogen：Synthetic peptide corresponding to aa1-100 at the internal region of human Bmi1.
