5-Hydroxytryptophan (5-HTP), also known as oxitriptan (INN), is a naturally occurring amino acid and chemical precursor as well as metabolic intermediate in the biosynthesis of the neurotransmitters serotonin and melatonin from tryptophan.
5-Hydroxytryptophan is decarboxylated to serotonin (5-hydroxytryptamine or 5-HT) by the enzyme aromatic-L-amino-acid decarboxylase with the help of vitamin B6.
This reaction occurs both in nervous tissue and in the liver.
5-HTP crosses the blood-brain barrier, while 5-HT does not
Since 5-HTP incresease levels of serotonin, it is used to treat depression, sleep disorders and pain.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (biotin-avidin/HRP staining): 1:1000-1:2000 in PBS, 0.3% Triton X-100Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in 0.09% sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes 5HTP. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Rt
Immunogen：5-HTP, coupled to BSA with paraformaldehyde.
