84-2654-21　Cyclic GMP (cGMP, Cyclic Guanosine Monophosphate) 100ul　298164

特徴

• Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP.
• Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC).
• Receptor activation of GC leads to the conversion of GTP to cGMP.
• Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme.
• Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
• Applications:Suitable for use in RIA.
• Other applications not tested.
• Recommended Dilution:RIA: 1:2000 bound 45-50% of the labeled (radioactive) nucleotide
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.