84-2654-18　Cyclic AMP (cAMP, Cyclic Adenosine Monophosphate) 20ul　298163

特徴

• Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals.
• The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors.
• They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules.
• Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP.
• The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins.
• Currently successful drugs in marketing have been developed to target these receptors.
• Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far.
• Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
• Applications:Suitable for use in RIA.
• Other applications not tested.
• Recommended Dilution:RIA: 1:5000 bound 45-50% of the labeled (radioactive) nucleotideOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.