84-2654-03　Beta-Defensin 2 (HBD-2) 100ug　298040

特徴

• The polyclonal antibody recognizes human beta-defensin 2 (hBD-2).
• hBD-2 is a cystein-rich cationic 41 amino acid antimicrobial peptide of 4-5kD.
• Human BD-2 is produced by epithelial cells upon stimulus by lipopolysaccharides and proinflammatory cytokines TNFalpha and IL1beta.
• Contact of keratinocytes with gram-negative bacteria results in rapid induction of hBD-2 protein.
• hBD-2 has been described as a dynamic component of the local epithelial defense system of the skin, intestinal and respiratory tract, where it functions by protecting surfaces from infection.
• Its local expression has been associated with skin lesions like psoriasis as well as infected lung epithelia of patients with cystic fibrosis.
• Furthermore, in inflammatory bowel disease (IBD), expression of hBD-2 is increased in patients with IBD compared to healthy persons.
• The NF-kB pathway has been recognized as a key component in the induction of hBD-2 expression, however, other studies have observed induction mediated by the mitogen-activated protein kinase (MAPK) pathways.
• Thus, increased expression of hBD-2 in epithelial cells is associated with the proinflammatory response.
• This is supported by the finding that the anti-inflammatory cytokines IL-10 and IL-13 downregulate the synthesis of hBD-2 in atopic dermatitis.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:50Western Blot: 1:50.
• The band size is 25kD for recombinant protein with tag and 5kD for the native protein
• Immunohistochemistry(Paraffin): 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.