Ricinus communis agglutinin 60(RCA60) is an extremely toxic glycoprotein consisting of two disulfide-linked chains of about 28,000 daltons and 32,000 daltons, termed A and B chain, respectively.
The B chain can bind to cell surfaces via galactose or N-acetylgalactosamine residues of membrane glycoconjugates and facilitates the transport of the lectin into the cell.
The A chain has an enzyme activity which can catalytically block protein synthesis and is so toxic that only a single molecule of A chain is required to kill a cell.
Alone, the A chain is incapable of entering the cell and is thus not toxic.
The A chain can be separated from the B chain and then linked by a cleavable disulfide bond to other proteins, such as antibodies.
These antibody-toxin hybrid molecules can be used to kill specific cells with the appropriate antigenic determinant exposed, such as tumor markers on surfaces of malignant cells.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 10mM sodium azide.
