Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport.
Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported.
In contrast to Ras or Rho, Ran is not associated with membranes.
Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs.
RanGDP involved in signaling.
Mammalian RanGAP is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000.
Detects a band of ~60kD Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：Xenopus
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes RanGAP1. Species Crossreactivity: Mouse, Human, Xenopus laevis
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mo Xe
Immunogen：Synthetic peptide mixture: CHWSDMFTGRLRPEI (aa82-96) and CPSPEKLVRMGPRRSA (aa434-448) from Xenopus laevis
