84-2592-73　［取扱停止］Ran (Androgen Receptor Associated Protein 24, ARA 24, ARA24, Gsp1, GTPbinding Nuclear Protein RAN, GTPase Ran, LPS, RAN Member RAS Oncogene Family, Ras like Protein TC4, Ras Related Nuclear Protein, RASL2 8, TC 4, TC4) 100ul　254265

特徴

• Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex.
• The Ran protein is also involved in control of DNA synthesis and cell cycle progression.
• Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).
• Mutations in Ran disrupt DNA synthesis Because of its many functions it is likely that Ran interacts with several other proteins.
• Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules.
• Ran could be a key signaling molecule regulating microtubule polymerization during mitosis.
• RCC1 generates a high local concentration of Ran-GTP around chromatin which in turn induces the local nucleation of microtubules.
• Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor.
• Polyglutamine repeat expansion in the AR is linked to Kennedys disease (X-linked spinal and bulbar muscular atrophy).
• Ran coactivation of the AR diminishes with polyglutamine expansion within the AR and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedys disease.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry(Paraffin): 5ug/ml.
• Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
• Western Blot: 1:500.
• Detects a band of ~26kD (predicted molecular weight: 26kD) Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.