Cellular tumor antigen p53 is a 50kD transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations.
Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Hybridoma:Sp2/0 Ag.14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.01% Thiomersal. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes cellular tumour antigen p53. Species Crossreactivity: Mouse, Rat, Bovine, Canine, Human, Monkey, Chicken, Chinese Hamster, Syrian Hamster
Isotype：IgG1
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Ch Hm Hu Mk Mo Rt
Immunogen：Purified p53-b-galactosidase fusion protein containing p53 sequence from aa14-389
