Nup53 encodes a recently identified nuclear pore protein.
Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope.
The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate.
Nup53p forms a complex with two other nucleoporins, Nup170p and Asm4p.
The complex is found on both the nuclear and cytoplasmic faces of the NPC, and associates with Pse1p, a member of a protein family implicated in nuclear protein import, via direct interaction between Nup53p and Pse1p.
Nup53p is structurally similar to Asm4p, and similar proteins sequences are found in several eukaryotes including human and other multicellular species.
Nup53p is not essential; deletion of Nup53 and ASM4 causes slow growth and altered subcellular distribution of Pse1p.
In a nup53 pse1 double mutant, NLS-containing proteins are mislocalized to the cytoplasm.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Detects a band of ~53kD Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Recognizes Nup53. Species Crossreactivity: Human, Rat, Saccharomyces cerevisiae
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein fusion protein (S. cerevisiae).
