84-2592-45　Nesprin 1 (8B, CPG2, Enaptin, MYNE1, Myocyte Nuclear Envelope Protein 1, Nesprin 1 Alpha, Nesprin 1, Nuclear Envelope Spectrin Repeat Protein 1, SCAR8, Spectrin Repeat Containing Nuclear Envelope 1, Synaptic Nuclear envelopeProtein 1, SYNE1, SYNE1B) 100ul　254171

特徴

• There are two genes encoding members of a new family of type II integral membrane proteins.
• Both are ubiquitously expressed and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein with the smaller isoforms being truncated at the N-terminus.
• These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat as they are characterized by the presence of multiple clustered spectrin repeats bipartite nuclear localization sequences and a conserved C-terminal single transmembrane domain.
• Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal TM domain-containing sequence essential for perinuclear localization.
• Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation.
• Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structural integrity.
• There are two genes encoding members of a new family of type II integral membrane proteins.
• Both are ubiquitously expressed and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein with the smaller isoforms being truncated at the N-terminus.
• These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat as they are characterized by the presence of multiple clustered spectrin repeats bipartite nuclear localization sequences and a conserved C-terminal single transmembrane domain.
• Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal TM-domain-containing sequence essential for perinuclear localization.
• Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation.
• Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structural integrity.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry(PFA fixed): Use at an assay dependent dilution.
• PubMed: 17159980 Use at an assay dependent dilution.
• Perfused with 4% PFA in PBS from (PubMed:17159980).
• Western Blot: 1:500-1:750.
• Detects a band of ~112kD.
• Immunocytochemistry/Immunofluorescence: 1:25-1:50.
• Fix cells with cold methanol: acetone 1:1 for 10 minutes on ice IHC-Fr: 1:25 -1:50.
• Fix with cold methanol: acetone 1: 1 for 10 minutes on ice Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.