84-2592-44　NEK2 (Serine Threonine Protein Kinase Nek2, HSPK 21, HsPK21, NEK 2, NEK2, NEK2_HUMAN, NEK2A, Never in Mitosis A-Related Kinase 2, NIMA (never inMitosis gene a) Related Kinase 2, NimA like Protein Kinase 1, NIMA Related Kinase 2, NimA Related Protein Kinase 2, NimA-like Protein Kinase 1, NimA-Related Protein Kinase2, NLK 1, Serine/Threonine Protein Kinase Nek2, Ser ...　254170

特徴

• NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2.
• Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2.
• These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression.
• Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation.
• Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides.
• NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5mg/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.