84-2592-41　LAP2 alpha (OIP5, 5730547N13Rik, Cancer/testis antigen 86, CT 86, CT86, LAP2alpha interactor 25, LINT 25, LINT25, MIS18beta, OIP 5, Opa Interacting Protein 5) 100ul　254151

特徴

• Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins.
• Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins lamin B receptor emerin and MANI three isoforms of lamina-associated polypeptide 1 (LAP 1) and several isoforms of LAP 2.
• Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus.
• The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha which are identical in their N-terminal 187aa constant region but differ in their C terminal.
• While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth.
• LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunocytochemistry and ChIP.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:2500.
• Detects a band of ~83kD.
• Immunocytochemistry/Immunofluorescence: 1:250 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.