An important part of the cell nucleus is formed by nuclear lamina.
Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane.
Two main subtypes of nuclear lamins can be distinguished ie A-type lamins and B-type lamins.
The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing ie lamin A lamin C and lamin Adel10 while the B-type lamins include two proteins arising from two distinct genes ie lamin B1 and lamin B2.
The nuclear lamins comprise a unique subclass of the intermediate filament protein family.
They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head a central rod of a-helices and a carboxyterminal globular domain.
Many biochemical and molecular features of lamins have been studied but their functions remain still largely undetermined.
One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus.
Besides interactions with the nuclear membrane and other intermediate filaments lamins interact with the nuclear chromatin.
Eukaryotic chromatin is organized into loops which are attached to the nuclear matrix.
This organization is thought to contribute to compaction of the chromatin and regulation of gene expression.
Lamins as part of the nuclear matrix may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.
Applications:Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100 Immunohistochemistry(Paraffin): 1:50-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human Lamin C. When tested against nuclear protein extracts from HeLa cells it reacts with a single band migrating at 65kD Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：FC IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A peptide made from the last 8aa of lamin C, including an N-terminal lysine as a linker (KHHVSGSRR), coupled to KLH
