84-2592-23　［取扱停止］APPL1 (Adaptor Protein Containing pH Domain, Adaptor Protein containing pH Domain PTB Domain and Leucinezipper Motif 1, Adaptor Protein containing pH Domain PTB Domain and Leucine zipper Motif, AKT2INTERACTOR, APPL 1, APPL1, DCC Interacting Protein 13 alpha, DIP 13 alpha, DIP13 alpha, DIP13alpha, KIAA1428, PTB Domain and Leucine zipper Motif 1, PTB Domain and Leuc ...　253931

特徴

• APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain, was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait.
• APPL1 binds to a number of cell surface receptors (TrkA, DCC, adiponectin, FSH) and intracellular signaling molecules (small GTPase Rab5, GIPC and inositol 5- phosphatase, suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades.
• APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500.
• Predicted molecular weight: 80kD.
• Immunocytochemistry/Immunofluorescence: 1:500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.