Indoleamine 2,3-dioxygenase (IDO) is an IFN-y inducible enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.
The gene encoding human IDO maps to chromosome 8p12-p11.
IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes.
IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs.
The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect.
Flow cytometric analysis indicates both mature and immature CD123-positive dentritic cells suppress T cell activity using IDO.
IDO-transfected cells co-cultured with T cells reduces T cell proliferation.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Indoleamine 2,3-dioxygenase 1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Indoleamine 2,3-dioxygenase 1.
