Predominantly present in the azurophilic granules of neutrophils and monocytes.
Elastase has a broad range of extracellular matrix substrates including elastin, proteoglycans, collagen and fibronectin.
The action of elastase is controlled by serine proteinase inhibitors.
Elastase, when released during inflammation, is rapidly bound by its two main inhibitors, alpha1-PI and alpha2-macroglobuline to form elastase-inhibitor complexes.
In addition mucosa secretions may contain the locally secreted elastase inhibitors elafin/SKALP and SLPI.
When secreted at sites of inflammation elastase can cause severe tissue damage.
An important role has been suggested for human elastase in various inflammatory disorders, including pulmonary emphysema, sepsis, arthritis, nephritis and certain skin diseases.
Elastase induces the production of IL-8 in human bronchial epithelial, a proces that occurs in part through TLR4.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
