84-2590-14　Polyglutamate chain (polyE) 50ug　227380

特徴

• Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin.
• Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin.
• Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation.
• These PTMs might have evolved to specifically regulate tubulin and microtubule functions.
• Polyglutamylation is a PTM that occurs when secondary glutamate side chains are formed on y-carboxyl groups of glutamate residues in a protein.
• Enzymes catalyzing polyglutamylation belong to the TTL-like (TTLL; Tubulin tyrosine ligase-like) family of glutamylases.
• Deglutamylases, the enzymes that reverse polyglutamylation, were identified within a novel family of CCPs (cytosolic carboxypeptidase).
• Subtle differences in polyglutamylation can be seen on diverse microtubules in different cell types.
• The functions of these modifications remain to be studied.
• However, its wide distribution strengthens the idea that it could be involved in fine-tuning a range of microtubule functions.
• Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:2000Immunohistochemistry (paraffin): 1:1000 Immunocytochemistry: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.