SH2D2A (SH2 domain-containing protein 2A; also TSAd and VRAP) is a 52kD protein best described as a multifunctional intracellular adaptor molecule.
It is expressed in activated CD4+ and CD8+ T cells, endothelial cells, and fibroblasts.
SH2D2A is found in both cytoplasm and nucleus, and has multiple binding partners.
After TCR activation, SH2D2A interacts with LCK, prolonging its activity.
It also binds to SMAD2 and SMAD3, and constitutively interacts with PI3K and PLCg, thus participating in VEGF:KDR signaling.
Human SH2D2A is 389 amino acids (aa) in length and contains one SH2 domain (aa 95-186) and a Pro-rich region (aa 187-389).
Phosphorylation occurs on Y39, Y216 and Y305, and on S217 and S296.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
AA Sequence:MEFPLAQICPQGSHEAPIPTFSTFQITDMTRRSCQNLGYTAASPQAPEAASSTGNAERAEEVPGEGSLFLQAETRAWFQKTQAHWLLQHGAAPAWFHGFITRREAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRHFLLAQLRDGRHVVLGEDSAHARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQDPNPQYSPIIKQGQAPVPMQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPSNPIYNEPDEPIAFYAMGRGSPGEAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQGPPLPHQPPPAWRHTLPHNLSRQVLQDRGQAWLPLGPPQ
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human SH2D2A.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length human SH2D2A, aa1-389 (NP_003966.1).
