84-2502-92　UBE2Q2 (Ubiquitin-conjugating Enzyme E2 Q2, Ubiquitin Carrier Protein Q2, Ubiquitin-protein Ligase Q2) (FITC) 100ul　U1000-93-FITC

特徴

• The ubiquitination pathway is known to regulate critical control points in multiple cellular processes by targeting proteins for activation, degradation, or localization at specific intracellular sites.
• The pathway involves an enzymatic cascade in which the first step activates ubiquitin by forming a high energy thiolester intermediate to ubiquitin activating enzyme (E1) that is subsequently transferred to members of an E2 ubiquitin conjugating enzyme superfamily.
• The E2 ubiquitin conjugating 45 enzymes function together with a larger superfamily of E3 ubiquitin ligases to transfer activated ubiquitin to specific cellular proteins.
• The existence of E2 and E3 superfamilies reflects the specificity of each E2-E3 cognate pair for a specific group of cellular substrates.
• UBE2Q2 is a potentially unique member of the ubiquitin conjugating enzyme superfamily that includes at least 17 phenotypically different subfamilies, many of which contain isozymes.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• For maximum recovery of product, 11c7entrifuge the original vial after thawing and prior to removing the cap.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.