84-2502-55　KDELR2 (ER Lumen Protein Retaining Receptor 2, ERD2-like Protein 1, ELP-1, KDEL Endoplasmic Reticulum Protein Retention Receptor 2, KDEL Receptor 2, ERD2.2) (FITC) 100ul　K0138-38-FITC

特徴

• Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment.
• Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S.
• cerevisiae.
• This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER.
• In yeast, the sorting receptor encoded by a single gene, ERD2, is a seven-transmembrane protein.
• Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described.
• KDELR2 was the second member of the family to be identified, and it encodes a protein which is 83% identical to the KDELR1 gene product.
• Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• For maximum recovery of product, 11c7entrifuge the original vial after thawing and prior to removing the cap.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.