White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure.
WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis.
Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate.
BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1).
UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs).
Both amino and C-termini are predicted to be cytoplasmic.
Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7; ~95% homology) mitochondrial uncoupling protein.
It is only 59% homologous with UCP1 found in brown adipose tissues.
UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle).
UCP2 may play a critical role in energy balance, body weight and thermoregulation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
AA Sequence:MVGFKATDVPPTATVKFLGAGTAACIADLITFPLDTAKVRLQIQGESQGPVRATASAQYRGVMGTILTMVRTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHASIGSRLLAGSTTGALAVAVAQPTDVVKVRFQAQARAGGGRRYQSTVNAYKTIAREEGFRGLWKGTSPNVARNAIVNCAELVTYDLIKDALLKANLMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSALGQYSSAGHCALTMLQKEGPRAFYKGFMPSFLRLGSWNVVMFVTYEQLKRALMAACTSREAPF
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human UCP2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length human UCP2, aa1-309 (NP_003346.2).
