Most mammalian species have multiple IFN-a subtypes.
Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression.
A recent study established a nomenclature for the murine IFN-a subtypes (van Pesch et al.
2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein.
In this study, Mu IFN-a4 was deemed to have average antiviral activity when compared with the potency of the other subtypes.
Mu IFN-a4 was initially cloned by Zwarthoff et al.
[(1985) Nuc.
Acids Res.
13(3) 791], and has been extensively studied.
It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3.
It may be that Mu IFN-a4, like IFN-b, has a priming function on cells, enabling the expression of other Mu IFN-a subtypes [Reviewed by Mesplède et al.
(2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J.
Exp.
Med.
202(4):461].
Thus, this interferon is among the first observed after viral infection.
Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al.
(2002) J.
Exp.
Med.
195(4):507]
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human IFNA4.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length human IFNA4, aa1-189 (NP_066546.1).
