84-2485-66　HLA-DRB5 (HLA Class II Histocompatibility Antigen, DR beta 5 Chain, DR beta-5, DR2-beta-2, Dw2, MHC Class II Antigen DRB5) (Biotin) 100ul　127933-Biotin

特徴

• HLA-DRB5 belongs to the HLA class II beta chain paralogues.
• This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane.
• It plays a central role in the immune system by presenting peptides derived from extracellular proteins.
• Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages).
• The beta chain is approximately 26-28 kDa and its gene contains 6 exons.
• Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
• Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities.
• Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
• DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5.
• The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted.
• There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• AA Sequence:MVCLKLPGGSYMAKLTVTLMVLSSPLALAGDTRPRFLQQDKYECHFFNGTERVRFLHRDIYNQEEDLRFDSDVGEYRAVTELGRPDAEYWNSQKDFLEDRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPARTQTLQHHNLLVCSVNGFYPGSIEVRWFRNSQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRAQSESAQSKMLSGVGGFVLGLLFLGAGLFIYFKNQKGHSGLHPTGLVS
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
• Note: Applications are based on unconjugated antibody.