Members of the F-box protein family, such as FBXL18, are characterized by an approximately 40aa F-box motif.
SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases.
F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
AA Sequence:MHAVPRGFGKKVRVGVQSCPPLLGPGVPPALLRVLVSAEEPALPGTPRADWVQLLLRHAPQRARHPQLAPTLQPRTECRGLGGGRHRPAGLPAAPDARTAAQRPYGLRAGQYRPAVPAVAVPVAGQPGHDGEGGVHARALRHVEALQAAEGPQAGAALLQRQRPVLPGAEPVPLAAAPVPGLSQRHPPARCRAGLHGSLPAGCHVPPVHRGVPRHLQEPAAVASPQLPGRAARVKRRHLPSAPRGPDRRHPGRPPGAPG
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human FBXL18.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length human FBXL18, aa1-259 (AAH13435.1).
