CDC2/CDK1, a CDC2/CDK -type protein kinase, regulates cell cycle transitions G1 to S and G2 to M.
CDC2/CDK1 is maintained in an inactive state by phosphorylation by kinases MYT1 and WEE1.
The active kinase, called the M-phase promoting factor, consists of a heterodimer with cyclin B, and final activation at G2/M results from CDC25 phosphatase-mediated dephosphorylation of CDC2/CDK1.
The drug Taxol has been shown to activate CDC2/CDK1 in MDA-MB-435 breast cancer cells, leading to cell cycle arrest at G2/M and to apoptosis.
CDC2 has also been implicated in neurofibrillary degeneration in Alzheimer's disease and other neurodegenerative diseases.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
AA Sequence:MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human CDC2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length human CDC2, aa1-297 (NP_001777.1).
