CD160 is a 27kD member of Ig superfamily of molecules.
It is expressed on select hematopoietic cell types, including CD56dimCD16+ cytotoxic NK cells, CD8+CD28 effector T cells, YdT cells, and restricted CD4+ T cells.
It is a receptor for HLAC molecules, and its engagement induces CD160+ NK cells to both secrete IFN-Y plus TNF-A, and initiate a cytotoxic program.
Human CD160 was originally identified as a 155aa pro-protein aa27-181.
It contains a 132aa mature region aa27-159 and a C-terminal pro-segment that is cleaved to create a GPI linkage.
The mature region possesses one V-type Ig-like domain aa27-122.
CD160 is found as a soluble, disulfide-linked 80kD multimer (likely trimer) that is generated by proteolysis of the GPI-linked form.
This 80kD form, plus others, are highly resistant to reduction.
There is also a 100-110kD multimeric transmembrane (TM) form that is associated with activated NK cells.
It contains a 55aa substitution for aa180-181, and shows a 20aa TM segment between aa163- 182.
The TM form appears to have a splice variant that lacks aa25-133.
Over aa27-159, human CD160 shares only 62% aa sequence identity with mouse CD160.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human CD160.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full-length human CD160, aa1-181 (AAH14465.1).
