Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin actin cytoskeleton and therefore play a role in cell motility, proliferation and intercellular contact.
Different isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue specific, developmentally regulated manner.
Most ankyrins are typically composed of three structural domains.
Ankyrin 1, the prototype of this family, was first discovered in erythrocytes but has also been found in brain and muscles.
Mutations in erythrocytic ankyrin 1 have been associated with hereditary spherocytosis.
Different isoforms of ankyrin 1 have been described.
Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
AA Sequence:MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human ANK1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length human ANK1, aa1-155 (AAH30957.1).
