Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL.
The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion, consistent with the belief that paxillin is involved in the regulation of cell morphology.
Additionally because of its SH3-binding domain, paxillin associates tightly with FAK and Crk in a manner that is independent of the extracellular matrix.
Although paxillin was initially detected in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
Human paxillin gene is mapped to chromosome 12q24.31.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
AA Sequence:MPDTFNSRSPSVEGSLWAVGTESQGRDWRHLPTITSELSGAPRCHTVPCAGSTALQEPGEPQGPPASPSCPEEALAATWEQPWASEVFGPERMPPSGAARSFQEVTEPAVVAVDRQAIFPDTWTLTEEHGLQQERPRPEPGRLGSSSPASVTTEQLGAKMTERGSVARPTQGPETPRSPEGTTEAATQDGKEQPELPCAMAMGTPSTTERISTSGQAGTSSHGCWAWPLAGLSVGRSSLTACSLPLCACLLGQKMLRHCGRT
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human PXN.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Full length recombinant protein corresponding to aa1-262 from human PXN
