84-2435-39　［取扱停止］POLR1D (DNA-directed RNA Polymerases I and III Subunit RPAC2, AC19, DNA-directed RNA Polymerase I Subunit D, RNA Polymerase I 16kD Subunit, RPC16, hRPA19, FLJ20616, MGC9850) (AP) 100ul　131581-AP

特徴

• DNA polymerase specifically involved in DNA repair.
• Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.
• Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts.
• Lacks 3'-5' proofreading exonuclease activity.
• Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• AA Sequence:MEEDQELERKISGLKTSMAEGERKTALEMVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRGTLPAVEPFQRGLNELMNVCQHVLDKFEASIKDYKDQKASRNESTF
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.