Zyxin is a low abundance phosphoprotein localized to focal adhesion plaques and is thought to perform regulatory functions at these regions.
The protein contains a number of proline-rich sequences as well as three LIM domains, zinc finger domains involved in protein binding.
Zyxin interacts with several other proteins at cell adhesion sites, including members of the CRP (cysteine-rich protein) LIM domain containing protein family.
The proline-rich domain of Zyxin associates with an SH3 domain of p95 Vav, but not with similar SH3 domains containing proteins such as GRB2 or PLCg.
Zyxin has also been shown to interact with the focal adhesion protein VASP and may assist in the targeting of VASP to focal adhesions, microfilaments and membrane regions of high dynamic activity.
Zyxin may contribute to the organization of the Actin cytoskeleton in mammalian cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ZYX. Species Crossreactivity: Human, mouse, rat
