ZWINT (ZW10 interactor), also known as KNTC2AP or HZwint-1, is a 277 amino acid protein that is involved in kinetochore function.
Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate the association between ZW10 and kinetochores.
Additionally, ZWINT is part of a kinetochore complex composed of proteins such as MIS12 (MIND kinetochore complex component) and PMF-1 (polyamine-modulated factor 1) that work in concert to ensure proper kinetochore formation and spindle checkpoint activity.
Defects in the gene encoding ZWINT are associated with the pathogenesis of Roberts syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ZWINT. Species Crossreactivity: Human
