The best studied of the immediate early genes is the c-Fos proto-oncogene.
Many of the signals inducing Fos expression act through a sequence located in the 5' flanking region of c-Fos, designated the serum response element (SRE).
The SRE is required for response to activators of protein kinase C and Fos growth-induced signals independent of protein kinase C.
Accumulating evidence argues that the SRE is a multifunctional element that may involve the action of multiple SRE-binding proteins.
These include the serum response factor (SRF) and the two less well characterized proteins, TCF p62 and BBF p62.
An SRE binding nuclear protein, designated SRE-ZBP, is a member of the C2H2 zinc finger family of proteins.
Like c-Fos, SRE-ZBP is serum-inducible in HeLa cells, although with slower kinetics.
Applications:Suitable for use in Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ZNF187. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 149-194 of Human ZNF187.
