ZNF174, also known as ZSCAN8 or AW-1, is a transcriptional repressor that belongs to the Krueppel C2H2-type zinc-finger protein family.
It is expressed in a number of different tissues, including small intestine, prostate, colon, spleen, pancreas, skeletal muscle, brain, heart, kidney and thymus, but it is most predominantly found in adult ovary and testis.
ZNF174 specifically acts to repress the promoter activities of PDGF-B and TGFbeta1.
ZNF174 localizes to the nucleus and contains three C2H2-type zinc fingers at the C-terminus and one SCAN domain near the N-terminus.
SCAN domains are found in a number of zinc-finger proteins and are characterized by a conserved region of 84 residues.
The SCAN domain seemingly regulates the association of proteins containing SCAN domains into noncovalent complexes and may also function as an underlying mechanism in selective oligomerization of these proteins.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ZNF174. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 168-228 of Human ZNF174.
