ZADH1 (zinc binding alcohol dehydrogenase,domain containing 1), also known as PTGR2 (prostaglandin reductase 2), PGR2 or 15-oxoprostaglandin 13-reductase, is a 351 amino acid cytoplasmic protein that belongs to the NADP-dependent oxidoreductase L4BD family.
Functioning as s a 15-oxoprostaglandin 13-reductase, ZADH1 catalyzes the conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2 in a NADPH-dependent manner.
ZADH1 overexpression has been found to repress PPARγ transcriptional activity and adipocyte differentiation.
Widely expressed, ZADH1 is found at highest levels in heart, kidney, liver, pancreas and prostate, with moderate levels found in brain, small intestine, lung, testis and skeletal muscle.
ZADH1 exists as a monomer, utilizes NADPH as a cofactor, and undergoes alternative splicing to produce two isoforms that are encoded by a gene located on human chromosome 14q24.3.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ZADH1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 228-273 of Human ZADH1.
