84-2413-18　［取扱停止］WT1-AS (Putative Wilms Tumor upstream neighbor 1 Gene Protein, WIT-1, Wilms Tumor-Associated antisense RNA, WT1-AS, WIT1) 50ul　226708

特徴

• Wilms’ tumor is a neoplasm of the kidneys that usually occurs in children and is characterized by the presence of abortive tubules and glomeruli surrounded by a spindled cell stroma.
• The 11p13 Wilms’ tumor locus consists of two coordinately regulated transcripts, WT1 and WIT-1, which are mutated in Wilms’ tumors.
• Wilms’ tumor upstream neighbor 1 (WIT-1) is encoded by an intron-less gene upstream of the Wilms’ tumor 1 (WT1) gene, which is is important for nephrogenesis and gonadal growth.
• The WT1 gene is bi-directionally transcribed from the same promoter region as WIT-1, which may function as an antisense regulator of WT1.
• WIT-1 and WT1 have the same temporal and cell-restricted expression pattern, although the expression of WIT-1 is less abundant.
• Methylation of the WIT-1 gene is implicated in hematologic malignancy of chemoresistant acute myeloid leukemia.
• Single nucleotide polymorphisms (SNPs) in the WIT-1 gene are significantly associated with focal segmental glomerulosclerosis.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.