The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis.
The Wnt family has two functional classes according to their biological activities; Wnts that signal through a Wnt-1/wingless pathway by stabilizing cytoplasmic beta-catenin, and Wnts that stimulate intracellular Ca2+ release and activate two kinases, CamKII and PKC, in a G protein-dependent manner.
Wnt-3a is an intercellular signaling molecule that mediates cytoskeletal reorganization and regulates hippocampal development.
Human Wnt-3a is 96% homologous to mouse Wnt-3a protein and 84% homologous to human Wnt-3 protein.
The human Wnt-3a gene clusters with the Wnt-14 gene at chromosome 1q42.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Wnt3a. Species Crossreactivity: Human, mouse, rat
