Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation.
The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion.
beta-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1.
Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids.
When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression.
Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Wnt-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 301-350 of Human Wnt-1.
