84-2412-97　［取扱停止］WNK1 (Erythrocyte 65kD Protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient Protein, MGC163339, MGC163341, p65, PRKWNK1, Prostate derived sterile 20 like Kinase, Protein Kinase lysine deficient 1, Protein Kinase lysine-deficient 1, Protein Kinase with no lysine 1, PSK, Serine/threonine Protein Kinase WNK1, Serine/threonine-Protein Kinase WNK1, With no K, W ...　226698

特徴

• The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250–300 amino acids organized in two domains.
• WNK kinases (With No lysine (K)) are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydro-phobic residues.
• WNK kinases contain a lysine upstream of the traditional position, within a glycine string.
• This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups.
• The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1.
• Human WNK1 maps to chromosome 12p13 and encodes a 2382 protein that is primarily expressed in heart, kidney, muscle and distal nephron.
• Human WNK3 maps to chromosome Xp11.21-p11.23 and encodes a protein that is primarily expressed in brain.
• Human WNK4 maps to chromosome 17q21-q22 and encodes a 1243 amino acid protein that is expressed in kidney.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.